Type of study Experimental system Experimental details References In vivo Rat Colitis rat model, Myeloperoxidase (MPO) activity González et al. [42] Sprague - Dawley rat, Equilibrium binding assays for Peripheral benzodiazepine receptors Heat shock protein 27kD expression Rimbau et al. [16] Carbon tetrachloride (CCl4)-induced lipid peroxidation Myeloperoxidase (MPO) activity Romay et al., [43] Male wistar rat Oxalate induced renal calculi formation Microscopic evaluation of urinary crystals Histopathology of rinal tissues Renal tubular damage assessment by urinary marker enzymes (alkaline phosphatase, acid phosphatase and γ-glutamyltransferase Farooq et al. [44] Male wistar rat Thioacetamide induced fulminant hepatic failure Lipid peroxidation and tryptophan measurement in brain tissue Glutathione peroxidase and Catalase activity estimation in brain homogenate Histopathology and electronmicroscopy of brain tissue Sathyasaikumar et al. [19] Hamster Male golden Syrian hamsters Body weight, food intake and organs weight measurement Measurement total cholesterol (TC) and HDL cholesterol (HDL-C) in plasma Immunoblotting of NADPH oxidase Riss et al. [45] Male golden Syrian hamsters Copper-induced LDL oxidation Measurement of enzymatic activities of SOD, CAT, and GSH-Px in liver Sheu et al. [46] C. elegans N2 wild type C. elegans Life span assay Pharyngeal pumping counting assay Paraquat sensitivity assay Sonani et al. [6] N2 wild type, CL4176 (Alzheimer’s disease model), mu86, e1370, hx546 mutants. Life span assay Pharyngeal pumping counting assay Stress resistance assay Paralysis assay Sonani et al. [40] In vitro Human Erythrocyte Normal human erythrocytes and plasma samples Azobis (2-amidinopropane) dihydrochloride (AAPH) induced oxidative stress Cytosolic glutathione measurement Lipid peroxidation assay Romay and González [47] Normal human erythrocytes Azobis (2-amidinopropane) dihydrochloride (AAPH) induced oxidative stress Haemolysis assay Benedetti et al. [48] Normal human erythrocytes Azobis (2-amidinopropane) dihydrochloride (AAPH) induced oxidative stress Haemolysis assay Structural morphological analysis by SEM Pleonsil et al., [49] Rat Cardiomyocyte Adult rat ventricular cardiomyocytes Cell viability assay Lactate dehydrogenase assay ROS measurement by H2DCF-DA and DHE fluorescence TUNEL assay Caspase-3 assay DNA laddering assay Khan et al. [50] Biochemical assays* ARSA, HRSA, PRSA Romay et al., [43] FRAP Soni et al. [5] SRSA, HRSA, H2O2-RSA, HOCl-RSA, PRSA, Iron chelating activity. Bermejo et al. [51] DPPH, HRSA,SRSA, NO-RSA, FICA, R Power Thangam et al. [52] DPPH, ABTS, FRAP, HRSA, SRSA, FICA, R Power Sonani et al. [6] *Abbreviations ARSA: Alkoxyl radical scavenging activity assay; HRSA: Hydroxyl radical scavenging activity assay; PRSA: Peroxyl radical scavenging activity assay; NO-RSA – nitrous oxide ion scavenging activity assay; H2O2-RSA: Hydrogen peroxide radical scavenging activity assay; HOCl-RSA: Hypochlorous acid radical scavenging activity assay; FRAP: Ferric ion reducing ability of plasma assay; DPPH: α, α –diphenyl- β -picrylhydrazyl ion scavenging activity assay; ABTS:  2’- azino - bis (3 - ethylbenzothiazoline - 6 - sulphonic acid) ion scavenging activity assay; FICA: Ferrous ion-chelating activity assay; R Power: Reducing power assay

Table 1: In vitro and in vivo anti-oxidant and radical scavenging activity of PBPs.