| Trial-1 |
Ammonium.AcetateBuffer+Methanol(50:50) |
| Trial-2 |
Phosphate Buffer+ACN(50:50) |
| Trial-3 |
Phosphate Buffer+ACN+Methanol(20:40:40) |
| Trial-4 |
Phosphate Buffer+ACN(40:60) |
| Trial-5 |
Methanol+ACN (80:20) |
| Trial-6 |
Methanol+ACN+Water |
| (Optimized trial) |
(80:10:10) |