Enzyme Nanoparticle Application References β-Glucosidase(BGL) from Aspergillusniger Ironoxide nanoparticles Biofuelproduction [83] Superoxide dismutase (SOD) NanoFe3O4 coated onagoldelectrodesurface Biosensors [84] α-Amylase Silica nanoparticles Formulation of detergentfor enhancing removal ofstarchsoils [85] Trypsin Nanodiamond prepared by detonation(dND) Proteolysis [86] Lysozyme Chitosan nanofibers Antibacterial [87] Mucorjavanicuslipase Nano-sized magnetite Solvent-free synthesis of 3-diacylglycerols [88] Lipases from C.rugosaand Pseudomonascepacia Zirconia nanoparticles Resolution of (R,S)-ibuprofenand (R,S)-1-phenylethanol, respectively [89] Horseradish peroxidase (HRP) Magnetitesilica nanoparticles Immunoassays [90] Alcohol dehydrogenase from T. brockii(TbADH) Goldandsilver nanoparticles Alcoholsynthesis [91] Cholesterol oxidase Fe3O4 nanoparticles Analysis of total cholesterol in serum [92] Haloalkanedehalogenase Silica coated iron oxide nanoparticle Production of fusion proteins containing dehalogenase sequences [93] Laccase Chitosan-magnetic nanoparticle Bioremediation of environmental pollutants [94] Keratinase Fe3O4 nanoparticles Synthesis of keratin [95] α-Amylase Cellulose-coated magnetite nanoparticles Starch degradation [96] Β-Galactosidase Con A layered ZnO nanoparticles Lactose hydrolysis [97] Lipase Fe3O4 nanoparticles Hydrolysis of pNPP [98] Glucose oxidase Thiolated gold nanoparticle Estimation of glucose levelup to 300 mg/ml [99] Lipase Polystyrene nanoparticle Aminolysis, esterification, trans-esterification [100] α-Chymotrypsin Polystyrene nanoparticles Proteolysis (cleaves peptide amide bonds) [101] Diastase Silica coated nickel nanoparticle Starch hydrolysis [102] Bitter Gourd Peroxidase (BGP) TiO2nanoprticles Phenol and dye removal [73] Cellulase TiO2nanoprticles Hydrolysis of Carboxy methyl cellulose [75] Alpha amylase TiO2nanoprticles Starch hydrolysis [72] Alpha amylase TiO2nanoprticles Starch hydrolysis and refolding [74] Trypsin TiO2nanoprticles refolding [74] Alpha amylase Silver nanoparticles Starch hydrolysis [103]

Table 2: Enzymes immobilized on nanoparticles and their biotechnological applications.