Figure 2: 4-15% PAGE analysis of chemically cross-linked toxin complex under denaturing condition. The cross-linking reaction was performed for 2 h (A) 4 h (B), at RT with 2:1 ratio and 16 h (C) at 4°C with 4:1 ratio of either BMB or BM (PEG)3 with toxin complex. Toxin complex proteins were visualized by staining with Coomassie Blue R-250 stain. Standard protein marker 10 (lane 1), unreduced toxin complex (lane 2), reduced toxin complex (lane 3), reduced toxin complex + BMB (lane 4); reduced toxin complex + BM (PEG)3 (lane 5).