Figure 3: 4-15% SDS-PAGE analysis of chemically cross-linked toxin complex under denaturing condition (A & B). The crosslinking reaction was performed at 4°C with 10:1 ratio of crosslinkers and TC. (A) Standard protein marker (lane 1), reduced toxin complex from Hall strain (lane 2), reduced toxin complex from PS5 strain (lane 3), reduced toxin complex from PS5 strain + BM (PEG)3 for 16 h (lane 4), 32 h (lane 5), and 40 h (lane 6). (B) Standard protein marker (lane 1), reduced toxin complex from Hall strain (lane 2), reduced toxin complex from PS5 strain (lane 3), reduced toxin complex from PS5 strain + TMEA for 16 h (lane 4), 32 h (lane 5), and 40 h (lane 6).