Figure 3: Xyloside initiated GAG chains. ASMCs from ApoE+/+ and ApoE-/- mice were treated with xyloside (0.5 mmol/l) under basal conditions in the presence of [35S]-SO4 (50 μCi/ml) for 24 hr. Secreted proteoglycans were isolated and concentrated. GAG chains were cleaved from proteoglycan core proteins via a β-elimination reaction and were separated by SDS-PAGE on 4–13% acrylamide gradient gels. Dotted lines indicate middle of band.