| Properties |
PET |
OFM |
Traditional PK from blood |
Microdialysis |
| measured drug concentration |
total (free, protein bound drug and metabolites) concentrations |
total (free and protein bound drug) concentrations |
total (free and protein bound drug) concentrations |
Only free (active) drug concentrations |
| invasiveness |
non-invasive |
minimally-invasive |
non-invasive |
minimally-invasive |
| necessity of radiolabeling |
labelled drugs needed |
no need of labeling |
no need of labeling |
no need of labeling |
| drug administration |
intravenous |
any route |
any route |
any route |
| size of the molecules measured |
no cut off value |
no cut off value |
no cut off value |
membrane dependent cut off value (<100 kDa) |
| sample preparation for bioanalysis |
no need (no sample) |
needed (unfiltered samples) |
needed (whole blood) |
no need (filtered samples) |
| costs |
high |
low |
low |
low |
| surgical skills |
no need |
needed |
no need |
needed |
| organ specificity |
yes |
yes |
no, systemic blood |
yes |
| number of subjects (animals or human) used |
low |
low |
high |
low |
| time frame |
hours |
hours to days |
only a very limited number of sampling pro animal |
hours to days |
