Densitometric
(A) MCF-7 cells were treated with β casein (54-59) peptide (NS-20μM). Change inBTF3 expression was shown by western blotting using anti-BTF3 antibody, loading was done as-Lane 1: control, Lane 2: Expression after 24 hr and Lane 3: Expression after 48 hr. Lower panel shows expression of α-Tubulin which was detected using anti-Tubulin monoclonal antibody to check equal loading of protein.
(B) Densitometric studies showing fold change in expression of BTF3 during treated conditions is presented in respect to control. Results are shown as mean fold change ± SEM (n=3) and change was considered significant at P ≤ 0.05 (*).
Figure 2: Effect of NS on BTF3 Expression.