(A) MCF-7 cells were treated with β-casein (54-59) peptide and its analogs (20μM) AN1, AN2 and AN3 for 48 hrs. Change in BTF3 expression was shown by Western Blotting using anti-BTF3 antibody Lower panel shows expression of α-Tubulin which was detected using anti-α-Tubulin monoclonal antibody to check equal loading of protein. (B) Densitometric studies showing fold change in expression of BTF3 during treated conditions is presented in respect to control. The values and error bars represent average and standard deviations of three independent set of experimentsand change was considered significant at P ≤ 0.05 (*) , P ≤ 0.01 (**) and P ≤ 0.001 (***).

Figure 4: NS and Analogs downregulates the expression of BTF3.