IL- 2 IFN-γ IL-4 IL-10 TNF-α
Treatment at IC-10 Concentration Reporter GFP mRNA exp. Reporter GFP mRNA exp. Reporter GFP mRNA exp. Reporter GFP mRNA exp. Reporter GFP mRNA exp.
PMA+ Ion 5.35±0.498 62.22±3.98 4.05±0.47 67.66±15.36 3.75±0.44 2.85 ±0.27 3.21±0.33 3.180±0.29    
P + I + CSA(10µg/ml) 1.48±0.26 4.46±0.48 1.13±0.02 3.60±0.58 1.11±0.004 0.62±0.10 1.13±0.03 0.36±0.05    
P + I +FK506 (8 µg/ml) 1.87±0.12 5.39±0.40 1.37±.021 3.4±2.8 1.38±0.01 0.259±0.002 1.33±0.084 0.57±0.005    
P + I +Pentoxifylline (27.8µg/ml) 4.77±0.57 63.7±6.63 3.791±0.59 63.82±6.519 3.205±1.11 3.12±0.49 2.80±0.54 4.41±0.685    
P + I +Chlorpyriphos (10µg/ml) 2.16±0.21 9.27±0.424 1.49±0.12 6.79±3.88 1.38±0.21 0.268±0.01 1.54±0.413 0.622±0.04    
P + I + Cypermethin (10µg/ml) 3.00±0.26 41.87±5.38 12.19±0.22 37.24±11.92 2.08±0.38 1.42±0.278 1.240±0.169 0.857±0.160    
P + I + Captan (1µg/ml) 3.49±0.28 43.7±5.69 2.54±0.26 45.8±2.69 2.52±0.54 1.44±0.28 2.11±0.05 2.9±0.28    
LPS                 4.87±0.51 77.81±10.65
LPS + CSA (10µg/ml)                 4.86±0.36 69.37±29.51
LPS + FK506 (8 µg/ml)                 4.710±0.25 76.24±19.17
LPS + Pentoxifilline (27.8µg/ml)                 2.01±0.24 30.51±3.58
LPS +Chlorpyriphos (10µg/ml)                 4.85±0.8 79.96±12.65
LPS + Cypermethrin (10µg/ml)                 4.9±0.32 80.94±7.9
LPS + Captan (1µg/ml)                 5.27±0.7 82.28±5.57
Table 3: Reporter gene and endogenous cytokine gene expression after treatment with immunosuppressant and chemicals at IC-10 concentration in activated cells.