| Strain and plasmid |
Relevant genotype |
Source |
| Saccharomyces cerevisiae |
|
|
| CEN.PK102-3A |
MATα ura3-52 leu2-112 |
[18] |
| BSX000 (Control) |
CEN.PK102-3A derivative; pJFE3 |
[11] |
| cel7AF(Wild type) |
CEN.PK102-3A derivative; {pJCF}/( Tr-cel7A-FLAG) |
[11] |
| mnn1Δ/cel7AF |
CEN.PK102-3A derivative; mnn1::loxP-KanMX-loxP; {pJCF}/(Tr-cel7A-FLAG) |
This work |
| mnn9Δ/cel7AF |
CEN.PK102-3A derivative; mnn9::loxP-KanMX-loxP; {pJCF}/(Tr-cel7A-FLAG) |
This work |
| och1Δ/cel7AF |
CEN.PK102-3A derivative; och1::loxP-KanMX-loxP; {pJCF}/(Tr-cel7A-FLAG) |
This work |
| mnn1Δ/mnn9Δ/cel7AF |
CEN.PK102-3A derivative; mnn1::loxP-KanMX-loxP; mnn9::loxP-KanMX-loxP; {pJCF}/(Tr-cel7A-FLAG) |
This work |
| mnn1Δ/och1Δ/cel7AF |
CEN.PK102-3A derivative; mnn1::loxP-KanMX-loxP; och1::loxP-KanMX-loxP; {pJCF}/(Tr-cel7A-FLAG) |
This work |
| MDS1/cel7AF |
cel7AF derivative; {pYX242MH}/(Tr-MDS1-HA) |
This work |
| mnn1Δ/och1Δ/MDS1/cel7AF |
mnn1Δ/och1Δ/cel7AF derivative; {pYX242MH}/(Tr-MDS1-HA) |
This work |
| Plasmids |
|
|
| pJFE3 |
TEF1p-PGK1t |
[35] |
| pJCF |
pJFE3; TEF1p-Tr-cel7A-FLAG-PGK1t |
[11] |
| pAJ401mds1 |
Tr-MDS1 |
[15] |
| pYX242 |
TPI1p-polyA |
[36] |
| pYX242MH |
TPI1p-Tr-MDS1-HA-polyA |
This work |
