Patient related factors Donor tooth related factors Recipient site related factors Clinical factors
•Better results in younger patients
•A patient free of major systemic and metabolic problems or specific habits (e.g., smoking)
•Good oral hygiene and a cooperative attitude.
1. Periodontal ligament (PDL)
•The presence of intact and vital PDL attached to the root surface •Preservation of vital PDL when the tooth is outside the mouth using physiologic salt water or milk or preservation liquids and as short a surgery time as possible
•Enhanced healing of the gingival tissue by placing a 1 mm band of PDL fibers on the root above the crest of bone
•A major factor in the formation of alveolar bone
•A chance of inadequate PDL development as an effective attachment with an impacted tooth (nonfunctioning tooth)
2. Healing of dental pulp

•The preservation of Hertwig’s epithelial root sheath (HERS)
•Healing of the dental pulp occurs until Moorrees tooth development stage 5
•When the diameter of the apical foramina is >1 mm, there is more than an 87% chance the dental pulp will heal
3. Continuation of root development
•Ideal timing of transplantation is when development of the donor tooth roots is 3/4 to 4/5 complete
4. Gingival adaptation
•Tight flap adaptation prevents bacterial invasion into the recipient socket
5. Root morphology
•Teeth with a single, cone-shaped root without concavity around the cervical area are most favorable.
•Bone width and height should be adequate to receive the donor tooth
•Better healing can be expected if the PDL tissue is still attached •Transplantation should be performed the day of transplantation or within 1 month after extraction
Surgery should be performed by a clinician with experience in such areas as Surgery should be performed by a clinician with experience in such areas as donor tooth extraction, preparation of the recipient site, and tissue management
Table 2: Successful healing factors associated with autotransplantation of teeth.