Figure 1: Schematic illustration of methods in the experimental group a) The extracted tooth was sectioned, and the tooth crown and contents of the root canal were removed, b) The remaining root was hemisectioned axially and trimmed to 4.0×3.0 mm, c) The trimmed root was placed in a dish with root-surface sides up and was incubated in α-MEM with 120 μg/ml Kanamycin Sulfate, 3.0 μg/ml Amphotericin B and 15% Fetal Bovine Serum