Figure 2: A: Concentration-response curve of pSS IgG anti M3 (●) and normal IgG anti M3 (■) on nitric oxide synthase (NOS) activity from rat submandibular gland.Basal value (b) corresponds to NOS activity after 60 min of incubation [254 ± 19 pmol/g tissue wet wt]. Values are mean ± SEM of five experiments in each group performed in duplicate. *P<0.0001 versus 5x10-5 M L-NMMA + pSS IgG anti M3. B: Preparations [white columns: normal IgG anti M3 (n IgG anti M3) and black columns pSS IgG anti M3] were incubated for 60 min with 1x10-8 M pSS IgG anti M3 in absence (b) or in presence (c) of 5x10-5 M L-NMMA and L-NMMA plus 1x10-4 M L-arginine (d). Basal values before the addition of any antibody or drugs (a). Values are mean ± SEM of five experiments in each group performed in duplicate. *P<0.0001 versus n IgG anti M3, **P<0.001 versus nIgG anti M3.