Figure 4: Effect of pSS IgG anti M3 (black column) on NOS activity in submandibular gland. The glands were incubated with 1x10-8 M pSS IgG anti M3 alone or in the presence of 1x10-4 M synthetic M3 peptide, 1x10-5 M S-Methyl-U and 5x10-6 M U-73122 (A), 1x10-4 M synthetic M3 peptide, 5x10-6 M L-NIO and 5x10-5 M verapamil(B) and 1x10-4 M synthetic M3 peptide, 5x10-6 M N-PL and 5x10-6 M TFP (C). Basal values (white column) without any additions and n IgG anti M3 were also shown.Values are mean ± SEM of six experiments performed in duplicate. *P<0.001 versus basal and n IgG anti M3, **P<0.001 versus pSS IgG anti M3 alone.