Figure 5: Effects of pSS IgG anti M3 in salivary glands. (A) Submandibular glands were incubated with increasing concentration of pSS IgG anti M3 alone (●) or in the presence of ODQ (1x10-7 M) (▲) and n IgG anti M3 (■). Values represent the mean ± SEM of five experiments performed in duplicate. *P > 0.0001 versus pSS IgG anti M3 + ODQ. (B) Action of pSS IgG anti M3 on cyclic GMP (cGMP) accumulation rat submandibular gland were incubated with 1x10-8 M of pSS IgG anti M3 alone or in the presence of different inhibitors: U-73122 at 5x10-6 M, verapamil at 5x10-5 M and TFP at 5x10-6 M. Results are mean ± SEM of four experiments performed in duplicate in each groups. *P<0.0001 versus basal and n IgG anti M3, **P<0.001 versus pSS IgG anti M3.