Figure 4: Bacterial DNA analyzed from sputum samples before and after treatment.A) Subjects provided a sputum sample for DNA analysis (pre water, black bars). They then rinsed with water and provided a second sample (post water, gray bars). There was no statistical difference within types of bacteria (n = 5). B) When the water rinse was replaced by CHX, there was a statistically significant decrease in the Pm bacterium. *indicated p < 0.05 (n = 5). C) The same test was conducted using the combination rinse and statistically lower bacterial levels were measured in several of the bacterium. Cr showed a statistically significant increase in bacteria. P < 0.05 (n = 9). Aa: Aggregatibacter actinomycetemcomitans, Pg: Porphyromonas gingivalis , Tf: Tannerella forsythia, Td: Treponema denticola, En: Eubacterium nodatum, Fn: Fusobacterium nucleatum/periodonticum, Pi: Prevotella intermedia, Cr: Campylobacter rectus, Pm: Peptostreptococcus micros, EC: Eikenella corrodens , Cs: Capnocytophaga species.