Figure 4: HSG co-transplanted DPSC demonstrated glandular structures with acinar- and duct-like structures. HSG only (1 million cells) or HSG and DPSC (1 million each) were injected in HA hydrogels subcutaneously ventrally to the submandibular salivary gland in Rag1 -/- mice (n =3 mice per group). (A-C) H&E staining demonstrated immature cancer-like cells with large nuclei in HSG hydrogel plugs. (D-F) H&E staining showed the duct- and acinar-like structures represented by their polarized nuclei in the hydrogel plugs containing HSG and DPSC. (G-I) PAS staining indicated some randomly positive cells (in pink) were found in the HSG transplants alone. (J-L) Clusters of PAS-positive acinar-like with some PAS-negative duct- like structures were present in the HSG+DPSC transplants. (E and K, insets, and L) Blood vessels were found at the interface of HSG and DPSC where the glandular structures were observed. Scale bars indicate 100 μm.