] Primers Sequence and Annealing.
Gene PrimersSequence AnnealingTemperature
FAAH Fw.5’-gtggatagcctggcattgtg-3’
Rev. 5’-gagtgggcatggtgtagttg-3’		 64°C
β-actin Fw.5’-ccgtaaagacctctatgcca-3’
Rev. 5’-aagaaagggtgtaaaacgca-3’		 58°C
GAPDH Fw.5’-aggtcggtgtgaacggatttg-3’
Rev. 5’-tgtagaccatgtagttgaggtca-3’		 65°C
18S Probe.5’-/6FAM/accggcgcaagacggaccaga/36TAMSp/-3’
Fw.5’-cgccgctagaggtgaaattc-3’
Rev.5’-cattcttggcaaatgctttcg-3’		 60°C
YWHAZ Fw.5’-acttttggtacattgtggcttcaa-3’
Rev. 5’-ccgccaggacaaaccagtat-3’		 57°C
Table 1: Primers Sequence and Annealing Temperatures for FAAH and Housekeeping Genes Utilized in Real Time PCR.
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