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Figure 1: Sex identification bands. Panel a) PCR amplification of CHD Z and CHD W genes using P2 – P8 primers [12] both sexes have a single band, and sex determination was not possible. Panel b) ARMS-PCR method with primers P2-NP-MP [3] showed individuals with two bands (female) and with one (males). M.- 100 bp Molecular size Marker (Promega, USA). |