Figure 3: Effects of temperature on hemolysis of SRBCs by Osteolaemus tetraspis Or Mecistops cataphractus. Incubation of 1% SRBCs (v/v) with 0.5% crocodile serum, diluted with normal saline, for 30 min resulted in a temperature-dependent hemolysis. The results are expressed as % of maximum hemolysis, relative to a positive control, and represent the results of four independent determinations.