Season SCP, °C Osmolality mOsmol Protein concentration, mg/ml Specific ice nucleating activity, °C/mg Survival at  -85°C, % Survival at -22°C, % Winter -10,5 ± 1,2 550± 25 117 ± 19 -0,089± 0,02 50 98 Spring -8,5 ± 0,8 310± 18 106 ± 11 -0,08± 0,015 0 93 Autumn -7,7± 0,2 150± 14 45 ±  26 -0,17± 0,085 0 77 Summer -7,8±0,15 432± 10 56,8 ± 15 -0,14± 0,066 0 0

Table 1: Seasonal variations in the SCP, osmolality, protein concentration, specific ice nucleating activity in the hemolymph of Upis ceramboides and survival of beetles at different freezing temperatures.