Treatment*Caffeine (ppm)   Egg Production Mean % Nymph Emergence(x̅± SEM)
N** Total x̅ ± SEM Week 1 Week 2
0 60 264 44.00 ±2.44 a 11.74 ± 5.06 94.70 ±1.82a
10 60 170 28.34 ± 4.51 b 10.59 ± 5.27 91.76 ±4.62a
20 60 176 29.34 ±4.36 b 14.77 ±6.94 97.16 ± 1.62a
30 60 164 27.34 ±3.23 b 10.37 ±5.20 93.29 ±4.67a
40 60 127 21.17 ± 2.38 c 11.81 ±10.78 92.13 ±2.94a
50 60 81 13.50 ± 7.01 d 8.64  ±6.81 81.48 ± 10.07a
*Each treatment had six replications
**Total numbers of bed bug females per treatment
Table 4: The number (and average per replication) of eggs laid by 60 female bed bugs in one week after consuming caffeine in reconstituted human blood, plus the percent bed bug nymph emerged at week one and two after eggs were laid. Different letters represent significant differences (p<0.05) between the various test concentrations.