"Display of Eno1p on the cell surface of BY4741/eno1 cells harboring the plasmid was observed by immunofluorescence microscopy. For quantitative analysis of the relative amounts of Eno1p displayed on the surface of yeast cells during cultivation in a liquid medium, the fluorescence of yeast cells bound with AlexaFluor488- labeled IgG was measured. Eno1p-displaying yeast cells were grown for oral administration to mice; cells 1 displayed a large number of Eno1p- fusion proteins on their surfaces.
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Citation: Shibasaki S, Karasaki M, Ueda M (2014) Combining Proteomic Strategies and Molecular Display Technology for Development of Vaccines against Candida albicans. J Proteomics Bioinform 7: 134-138."
Last date updated on July, 2014