| Stem cell markers |
Sequences
(forward & reverse) |
Base pair(bp) |
Annealing Temperature |
References |
| Oct4 |
5’- CGACCATCTGCCGCTTTGAG -3’
5’- CCCCCTGTCCCCCATTCCTA-3 |
577 |
60°C/1 min |
Henderson JK, et.al. 2002 |
| Sox2 |
5’-GGCAGCTACAGCATGATGC-3’
5’- TCGGACTTGACCACCGAAC-3’ |
236 |
60°C/30 sec |
Bhatia B, et.al. 2011 |
| Nanog3
|
5’-CTG TGA TTT GTG GGC CTG A-3’
5’-TGT TTG CCT TTG GGA CTG GT-3’ |
151 |
56°C/30sec |
Nettersheim D,
et.al. 2011 |
| MTHFR C677T |
5’-TGA AGG AGA AGG TGT CTG CGG GA-3’
5’-TGA GAG TGG GGT GCA GGG AGC TT-3 |
195 |
58°C/1min |
Frosst P,
et.al. 1995 |
