Primers Forward CTTTGGTGAGATCCATTGACCTCAA Reverse AATCTTTGAGACCAAGTTCCAGGTG Reporter dye Probe designed for the detection of thep.R201H FAN–mutatedallele TCGCTGCCATGTCC VIC–wildtype allele TCGCTGCCGTGTCC

Table 2: Designed primers and probes for the detection of the gsp mutation.