| Sl.No. |
Primer |
Length (bp) |
Tm(°C) |
Ta(°C) |
GC (%) |
Sequence(5’→3’) |
Position of amplicon (nt) |
| Start |
End |
| 1 |
Left primer |
20 |
60.04 |
59.00 |
50.00 |
5‘CCTCATTGGGTCAGGAAGAA 3’ |
94 |
113 |
| Right primer |
20 |
60.07 |
59.00 |
55.00 |
5‘GGATCTCAGCCACCCACTTA 3’ |
334 |
315 |
| 2 |
Left primer |
20 |
59.03 |
59.00 |
55.00 |
5‘GACCAAGTTCAGGGAGACGA 3’ |
120 |
139 |
| Right primer |
20 |
59.83 |
59.00 |
55.00 |
5‘CAAGCTCGCGTAGTACAGGT 3’ |
627 |
608 |
| 3 |
Left primer |
20 |
59.55 |
59.00 |
55.00 |
5‘GGATCAAGCAGGAGATGAGC 3’ |
08 |
27 |
| Right primer |
20 |
59.83 |
59.00 |
55.00 |
5‘AAGCTCGCGTAGTACAGGTC 3’ |
626 |
607 |
