Figure 2: HLA typing of the family and the inheritance of haplotype (A). Study of the HLA class I (A, B, and Cw) and class II (DRB1) gene polymorphisms was conducted at high resolution by sequence-based typing (SBT) assay using commercial class I and class II sequencing kits (Abbott Molecular AlleleSEQR HLA-SBT ). Sequencing was carried out on a 3130 Genetic Analyzer (Applied Biosystems). Assign SBT v 3.5+ software (Conexio Genomics, Applecross, Australia) used the allele assignment to detect the heterozygous positions within each electropherogram and assess the typing on the basis of an alignment of the processed sequence with an updated HLA sequence library. (B) Measurements of cytokine levels were carried out using the Multi-Analyte Profiler ELISArray Kit (Qiagen) according to the manufacturer’s instructions. Three fourteen and three twelve years old females and three eleven years old males were selected as controls. Results are reported as absorbances at 450 nm. Statistical analyses were performed using the statistical package for the social sciences (SPSS) version 11.0 (SPSS, Tokyo, Japan). A Mann-Whitney test for non-parametric data was used to compare media. *p =0.011 for IL6; *p =0.035 for IL1β .