Lane 1: molecular weight marker.
Lane 2: shows no amplification (Normal healthy control) with HbQIndia mutant primer.
Lane 3: shows amplification with HbQIndia mutant primer (HbQIndia heterozygote).
Lane 4: No template control
|
| Figure 2: PCR-ARMS amplifications of the α 1 gene with mutant primer set for
HbQIndia in a 2% agarose gel. 766 bp product is ARMS internal control fragment
and 370 bp product is HbQIndia specific. Lane 1: molecular weight marker. Lane 2: shows no amplification (Normal healthy control) with HbQIndia mutant primer. Lane 3: shows amplification with HbQIndia mutant primer (HbQIndia heterozygote). Lane 4: No template control |
