Figure 2: Gene profiling of stem cell markers and self-renewal genes in the tumorspheres derived from IMCE-Ras cells. (A) RT-PCR analysis was performed to obtain the gene expression profiles in the tumorspheres and monolayer adherent cells. (B) Real-time PCR was performed to evaluate the relative expression levels of stem cell marker genes in the tumorspheres compared to monolayer cells. ML, monolayer; TS, Tumorsphere.