Table 1

Gene	Primer sequence(5’→3’)		Product length (bp)	Annealing temperature
AMXY	F AMXY	5’-6-FAM-CTG ATG GTT GGC CTC AAG CCT-3’		58°C
AMXY	R AMXY	5’-GGT GGC GGC TGT TGC TGC TGC TG-3’	432	58°C
SRY	F sY14	5’-GAA TAT TCC CGC TCT CCG GA-3’		60°C
SRY	R sY14	5’-GCT GGT GCT CCA TTC TTG AG-3’	472	60°C
TSPY	F TSPY	5’-CCT TTC ATC CCA ACC TTT ATT TCC A-3’		62°C
TSPY	R TSPY	5’-GCA GTC ATG TTC AGC CAA ACA GC-3’	273	62°C
DDX3Y	F DBY	5’-ATC GAC AAA GTA GTG GTT CC-3’		58°C
DDX3Y	R DBY	5’-AGA TTC AGT TGC CCC ACC AG3’	688	58°C
	F sY114	5’-TGC ACT CAT GGA GAC AAC AG-3’
HSFY	R sY114	5’-AAC CAG GGT TTT CAC TGA AA-3’	1450	60°C

Table 1: Sequences of the primers used in four PCR reactions and their annealing temperatures [19-22].