Parameter Quantitative Assay Mobile phase 5.0 g sodium 1-heptanesulfonate monohydrate in 250 ml HPLC grade water, 50 ml glacial acetic acid and 650 ml HPLC grade MeOH. Gradient 100% mobile phase A Column C18, 5µ, 4.6 x 150 mm Guard Column C18, 4 × 3 mm Flow Rate 2.0 ml/min Run time 20 min Injection Volume 10 µL Column temperature 20°C Detection Wavelength 330 nm Autosampler Temperature 6°C

Table 1: Instrument set-up and parameters.