Ovarian stage Histological description Oocyte diameter*(μm)
I – Immature/recovering (previtellogenic stage) Thick ovarian wall with centrally located germanium surrounded by number of oocytes; cells are spherical or oval in shape and arranged at the periphery of oocytes; ovary consists of primary and secondary oocytes; follicle cells are surrounded by bigger oocytes 30 – 90 (53 ± 9.2)
II – Developing (vitellogenic stage I) Oocytes consist of a band of yolk globules located peripherally with a prominent nucleus and nucleolus; oocytes are enveloped by follicle cells. 90 – 120 (110 ± 8.8)
III – Mature (vitellogenic stage II) Peripherally located yolk globules migrate towards the centre of oocytes. 120 – 170 (162 ± 11.1)
IV – Ripe (vitellogenic stage III) Large accumulation of yolk globules occupying the entire oocyte; disappearance of nucleus and nucleolus; fine inter dispersed protein globules in between the large lipid yolk globules; appearance of disintegrated follicle cells around oocytes. 170 – 210 (198 ± 8.9)
V – Spent ovary Ovary shows several post-ovulatory follicles along with unextruted ova. Atretic oocytes also present. 50 – 100 (71 ± 12)
Values in the parentheses are mean ± S.D. *n=12 crabs (360 oocytes)
Table 3: Histological description and diameters of the oocytes of the different stages of ovarian development in the crab O. senex senex.