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Figure 4: SMR is dispensable for viral replication and CD4+/CD3+ T cell depletion was reduced in HIVNefsmr5a infected PBMCs. (A) 4.0 X10^6 PBMCs from three HIV-1 seronegative donors were infected with HIVwt, HIVdsNef, or HIVsmr5A virus and assayed by ELISA for changes in p24 release in culture medium at 2-day intervals. p24 ELISA analysis revealed similar viral replication kinetics for HIVwt and HIVNefsmr5a viral clones for donors 1 and 2. (B) 4.0 X 10^6 PBMCs from nine HIV-1 seronegative donors were infected with HIVwt, HIVdsNef, or HIVsmr5A virus. Day 12 post infection, cells were harvested and analyzed for the percentage of CD3+ lymphocytes expressing CD4 by flow cytometry. The percentage of CD3+ lymphocytes expressing CD4 was significantly higher in HIVNefsmr5a infected cultures relative to HIVwt. Values reflect the means of three independent experiments with three different donors used per experiment. Error bars represent SEM of the mean. Differences between groups were determined using an unpaired student t-test *p<0.05, **p<0.01, ***p<0.001.