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Figure 3: (A) Structural model of mite allergen Der f 2. Der f 2 has three disulfide bonds (Cys21-Cys27, Cys73-Cys78 and Cys8-Cys119). The solid lines show the disulfide bonds. rDer f 2 is comprised of 130 amino acids, and its deduced molecular weight is 15 kDa. A methionine is added at the N-terminal of rDer f 2. Peptide mapping analysis of rDer f 2 (B). Samples derived from non-reduced treatment and reduced treatment were analyzed. Peak A was derived from a Cys21 –Cys27 disulfide bond-related peptide fragment, peak B from a Cys73 –Cys78 disulfide bond-related peptide fragment, and peak C from a Cys8 – Cys119 disulfide bond-related peptide fragment. (C) Amino acid sequences of peaks A, B, and C. The solid lines show the disulfide bonds. |