Components Volume mg/ 25 ml
NaCl 167.5 mg/ml
KCl 5.8 mg
NaHCO3 55 mg
BSA (Fatty Acid Free) 75 mg
Hemi-Ca Lactate 13.8 mg
Pyruvate 1 mg
L-Glutamine 3.8 mg
Basal Medium Eagle (BMA) (50X Stock) amino acids 500 µl
Minimum Essential medium (MEM) amini acids (100X stock) 250 µl
Gentamycin 12.5 µl
EGF 25 µl
ITS 5 µl
Table 4: Composition of CR1aa.