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 Research Article Open Access 
Pharmacokinetics of Casiopeína IIgly in Beagle Dog: A Copper Based Compound with Antineoplastic Activity
1Facultad de Química, Departamento de Farmacia, Universidad Nacional Autónoma de México, 04510 México D.F., México.

2Facultad de Química, Departamento de Química Inorgánica y Nuclear, Universidad Nacional Autónoma de México, 04510 México D.F., México
*Corresponding author: Lena Ruiz-Azuara, Ph.D. Facultad de Química.
Departamento de Química Inorgánica y Nuclear, Universidad Nacional
Autónoma de México, 04510,
Tel/Fax: (5255) 56223529
E-mail: [email protected]
 
Received January 27, 2010; Accepted March 22, 2010; Published March 22, 2010
Citation: Cañas-Alonso RC, Fuentes-Noriega I, Ruiz-Azuara L (2010) Pharmacokinetics of Casiopeína IIgly in Beagle Dog: A Copper Based Compound with Antineoplastic Activity. J Bioanal Biomed 2: 028-034. doi: 10.4172/1948-593X.1000018
 
Copyright: © 2010 Cañas-Alonso RC, et al. This is an open-access articldistributed under the terms of the Creative Commons Attribution License, hich permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 
Abstract
Casiopeína IIgly is a mixed chelate coordination complex with copper core that have demonstrated high antineoplastic activity in vitro and in vivo. In the present work, a developed and validated method for measurement of Casiopeína IIgly in beagle dog and its application in a pharmacokinetic study is reported. The analyte was isolated from blood by solid-phase extraction using Strata X cartridges. The analysis was carried out on a Synergy Polar-RP column (30 X 2.0 mm) using an isocratic elution and MeOH/ HFBA 0.1% (4:6) as the mobile phase. An Agilent LC/ MSD Trap VL equipped with an ionization electrospray source, was operated in selective ion storage (SIS) usingstable ion [Cu(II)(F7C3COO) 4,7-dimethyl phen ]+ with 484 m/z for quantification result of ESI reaction betweenCasiopeína IIgly and HFBA. The method demonstrated to be linear (r = 0.9992) in the range from the 0.1 to 15 μg/ml with a limit of detection (LOD) of 50 ng/ml. All the parameters of validation such as selectivity, accuracy, precision and recovery were within the required limits. Pharmacokinetics assay was carried out at 2 doses, indicated a high elimination rate.
 
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