| Research Article |
Open Access |
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| Sodium Deoxycholate Micelles Activated Separation of Coexisting Fivenucleobases by High-performance Thin-layer Chromatography |
| Ali Mohammad*, Sameen Laeeq and Abdul Moheman |
| Analytical Research Laboratory, Department of Applied Chemistry, Faculty of Engineering & Technology, Aligarh Muslim University, Aligarh, India |
| *Corresponding author: |
Dr. Ali Mohammad,
Analytical Research Laboratory,
Department of Applied Chemistry,
Faculty of Engineering & Technology, Aligarh
Muslim University, Aligarh-202 002, India,
Fax: +91-571-2702758,
E-mail: alimohammad08@gmail.com |
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| Received March 24, 2010; Accepted April 25, 2010; Published April 25, 2010 |
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| Citation: Mohammad A, Laeeq S, Moheman A (2010) Sodium Deoxycholate Micelles Activated Separation of Coexisting Five-nucleobases by High-performance Thin-layer Chromatography. J Bioanal Biomed 2: 055-059. doi: 10.4172/1948-593X.1000022 |
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| Copyright: © 2010 Mohammad A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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| Abstract |
| A novel high-performance thin-layer chromatographic method has been developed for the resolution of fi ve-coexisting nucleobases (adenine, guanine, cytosine, thymine, and uracil). The nucleobases were separated on aluminum-backed
cellulose 60 F254 plates with the aid of 5.0% aqueous sodium deoxycholate (NaDC)-acetonitrile (AcN), 1:3 (v/v) as
mobile phase. All the nucleobases were viewed on HPTLC plates under 254nm UV light. The order of RF value given
in parentheses was guanine (0.12) < adenine (0.44) < cytosine (0.50) < uracil (0.72) < thymine (0.84). The effect of
pH (acidity or basicity) of the mobile phase on the retention of individual nucleobases was examined. Furthermore, the
effect of interference of mono- (Li+, Na+), and bivalent (Mg2+ Ba2+, Co2+, Ni2+, Cu2+, Zn2+, Cd2+, Pb2+) cations; mono-
(Br -, CH3COO-, NO3 -) , and bivalent (CO3 2-, IO4 -, SO4 2- MoO4 2-) anions, and complexing ligands (urea, and EDTA) on
the retention behavior of nucleobases were also assessed. The chromatography of nucleobases was also performed
on silica 60 F254, RP-18 F254, and kieselgel 60 F254 HPTLC plates. These TLC plates failed to separate the coexisting
purines and pyrimidines. The detection limit of all nucleobases on cellulose 60 F254 layers was 5.4 × 10-2 μg spot-1.
The proposed method is rapid, easy, and reliable. It can be applied for routine analysis of DNA, and RNA nucleobases. |
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