| Research Article |
Open Access |
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| Bioencapsulation of Florfenicol in Brine Shrimp, Artemia
Franciscana, Nauplii |
| Irja Sunde Roiha1, Erling Otterlei2 and Ole Bent Samuelsen1,3* |
1Institute of Marine Research, P.O. Box 1870 Nordnes, N-5817 Bergen, Norway
2SagaFjord Sea Farm AS, Eldøyane 200, 5411 Stord, Norway
3Section of Pharmacology, Institute of Internal Medicine, University of Bergen, Armauer Hansens Hus, 5021 Bergen, Norway |
| *Corresponding author: |
Dr. Ole Bent Samuelsen,
Institute of Marine Research,
P.O. Box 1870 Nordnes,
N-5817 Bergen, Norway,
Fax: +47 55 23 85 31
E-mail: ole.samuelsen@imr.no |
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| Received May 14, 2010; Accepted June 25, 2010; Published June 25, 2010 |
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| Citation: Roiha IS, Otterlei E, Samuelsen OB (2010) Bioencapsulation of
Florfenicol in Brine Shrimp, Artemia Franciscana, Nauplii. J Bioanal Biomed 2: 060-
064. doi:10.4172/1948-593X.1000023 |
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| Copyright: © 2010 Roiha IS, et al. This is an open-access article distributed under
the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original author and
source are credited. |
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| Abstract |
| The brine shrimp Artemia franciscana is one of the most common live-feed organisms for use in the larval culture
of marine fish production. Bioencapsulation of florfenicol, an antibacterial agent, in Artemia nauplii was investigated as
a potential carrier for this drug to marine larvae. Florfenicol was delivered directly to the organisms as particles, and
the doses ranged from 100 to 2000 mg/l. Analysis of florfenicol concentrations in Artemia sp. nauplii were performed
using high performance liquid chromatography (HPLC). The uptake of florfenicol in Artemia nauplii increased with
particle size, dose, and exposure time, obtaining the highest concentration of 5.02 ng/nauplius, using a dose of 300
mg/l AQUAFLOR premix and 10 min exposure time. However, to obtain reproducible results, an enrichment time of at
least 60 min is recommended. |
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