| Nominal concentration (ng/mL) |
Stability |
Mean ± S.D.a
n = 6 (ng/mL) |
Precision b
(%) |
Accuracy c
(%) |
|
40
|
0 h (for all) |
39.05 ± 2.93 |
7.50 |
97.63 |
| 20 h (Auto-sampler) |
37.33 ± 1.45 |
3.89 |
95.60 |
| 4 h ( bench top) |
42.12 ± 1.56 |
3.71 |
107.85 |
| 3rd freeze-thaw |
42.07 ± 2.25 |
5.34 |
107.73 |
| 15 day at -80ºC |
42.27 ± 1.87 |
4.42 |
108.24 |
| |
|
|
|
|
| 1600 |
0 h (for all) |
1548.33 ± 43.09 |
2.78 |
96.77 |
| 20 h (Auto-sampler) |
1620 ± 71.27 |
4.40 |
104.63 |
| 4 h ( bench top) |
1546 ± 75.37 |
4.87 |
99.85 |
| 3rd freeze-thaw |
1544 ± 83.55 |
5.41 |
99.72 |
| 15 day at -80ºC |
1551.67 ± 64.32 |
4.15 |
100.22 |
a Back calculated plasma concentrations.
b Expressed as % R.S.D. (S.D./mean) × 100.
c Calculated as (mean determined concentration/nominal concentration) × 100. |
