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 Research Article Open Access 
Determination of Curcumin in Rat Plasma by Liquid–liquid Extraction using LC–MS/MS with Electrospray Ionization: Assay Development, Validation and Application to a Pharmacokinetic Study
Pharmacokinetics and Metabolism Division, Central Drug Research Institute, Council of Scientific and Industrial Research (CSIR), Lucknow 226001, Uttar Pradesh, India
*Corresponding author: Dr. Wahajuddin W
Pharmacokinetics and Metabolism Division
Central Drug Research Institute
CSIR, Lucknow 226001
Uttar Pradesh, India
Tel: +91-522-2612411-18 (Ext-4377)
Fax: +91-522-2623405
E-mail: [email protected], [email protected]
 
Received July 23, 2010; Accepted August 23, 2010; Published August 23, 2010
Citation: Singh SP, Wahajuddin W, Jain GK (2010) Determination of Curcumin in Rat Plasma by Liquid–liquid Extraction using LC–MS/MS with Electrospray Ionization: Assay Development, Validation and Application to a Pharmacokinetic Study. J Bioanal Biomed 2: 079-084. doi:10.4172/1948-593X.1000027
 
Copyright: © 2010 Singh SP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 
Abstract
A simple, specific and rapid LC–MS/MS method has been developed and validated for the estimation of curcumin in rat plasma, using biochanin as internal standard (IS). The assay procedure involved liquid–liquid extraction of curcumin and IS from rat plasma. The recovery of curcumin and IS in rat plasma were 87.62 and 88.25%, respectively. The resolution of peaks was achieved with 0.01 M ammonium acetate (pH 5.5):acetonitrile (10:90, v/v) on a Supelco Discovery C18 column. The total chromatographic run time was 4 min. Specificity and matrix effect on ionization was determined and found that method was specific and there was no significant matrix effect. The method was proved to be accurate and precise at linearity range of 10–2000 ng/mL with a correlation coefficient (r) of =0.995. The MS/MS ion transitions monitored were 367?217 for curcumin and 283?268 for IS. The intra- and inter-day assay precision ranged from 2.79 to 8.20% and 4.15 to 7.76%, respectively; and intra- and inter-day assay accuracy was between 92.83–107.83% and 93.92–104.26%, respectively. Practical utility of this LC–MS/MS method was demonstrated in a pilot pharmacokinetic study in male Sprague–Dawley rats following intravenous administration of curcumin.
 
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