| Research Article |
Open Access |
|
| Rapid and Specific Approach for Direct Measurement of Topiramate in Human Plasma by LC-MS/MS: Application for Bioequivalence Study |
| S. R. Kuchekar1*, M. L. Kundlik1 and B. H. Zaware2 |
| 1Padmashri Vikhe Patil College, Pravaranagar, Loni Kurd, Pin-413713, Dis- Ahmednagar, Maharastra State, India |
| 2New Arts, Commerce and Science college of Ahmedanagar-414 001, Maharashtra State, India |
| *Corresponding author: |
Dr. S. R. Kuchekar
Padmashri Vikhe Patil College
Pravaranagar, Loni Kurd
Pin-413713, Dis- Ahmednagar
Maharastra State, India
Tel: +91-02422-273425
Fax: +91- 02422-273426
E-mail: hyang@mmc.edu |
|
| |
| Received August 08, 2010; Accepted September 09, 2010; Published September
09, 2010 |
| |
| Citation: Kuchekar SR, Kundlik ML, Zaware BH (2010) Rapid and Specifi c
Approach for Direct Measurement of Topiramate in Human Plasma by LCMS/
MS: Application for Bioequivalence Study. J Bioanal Biomed 2: 107-112.
doi:10.4172/1948-593X.1000032 |
| |
| Copyright: © 2010 Kuchekar SR, et al. This is an open-access article distributed
under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the
original author and source are credited. |
| |
| Abstract |
A rapid liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-MS/MS) method
was developed and validated for the quantification of topiramate in heparinized human plasma. The plasma samples
were prepared by solid phase extraction (SPE) method without drying and then reconstitution. Topiramate and the
topiramate d12 (Internal Standard IS) were chromatographed on a Betasil C18 column at a flow rate of 0.5 ml/min. The
total run time was 1.80 min. An electrospray ionization interface was selected for ionization of analyte and IS. The mass
transition [M-H] ions used for detection were m/z 338.10→78.20 for topiramate, m/z 350.40→90.10 for IS. The method
was linear in the concentration range of 10–4200 ng/ml with r ≥0.9982. Recovery of topiramate and IS ranged from
78.20 to 87.74%. The validated method has been successfully used to analyze human plasma samples for application
in 100 mg fasted pharmacokinetic studies.
|
| |
|
This Article |
DOWNLOAD |
|
Services |
|
Google Scholar |
|
Pub Med |
|
|
