PARAMETERS HQC MQC LQC LLOQ QC Nominal Conc. 3605.58 2098.45 25.181 10.576 Mean 3589.69 2083.77 23.542 9.166 SD (±) 18.1303 8.6949 1.1647 0.9706 CV (%) 0.5 0.4 4.9 10.6 Accuracy 99.6 99.3 93.5 86.7

Table 3b: Precision and Accuracy studies of APT samples (ng/ml). LLOQQC – Lower limit of quantification Quality control samples, LQC - Lower Quality Control, MQC - Medium Quality Control, HQC - Higher Quality Control, SD - Standard Deviation and CV - Coefficient of variance.