Figure 4: Amplification of FVL (G1691A) using primers for mutant A alleles (lanes 1,3,5) and normal G allele (lanes 2,4,6) showing positive bands (152bp) in lane 2, 3,4,5 with negative bands in lanes 1,6 indicating G/G normal genotype in case 1 (lanes 1,2), G/A heterozygous genotype in case 2 (lanes 3,4), A/A homozygous mutant genotype in case 3 (lanes 5,6) and. M indicates molecular marker, Bands of size 250 bps belongs to F IX used as internal controls.