Figure 5: Splenic cytokine producing cells and pancreatic-draining lymph node specific Foxp3 expression. A-C. Two weeks following BM-DC treatment, 3x105 spleen cells were re-stimulated with GAD217-236 peptide for 4 days (n=3). Total spot forming units (SFU) were enumerated for the indicated cytokines with an automated spot counter. D,E. Two weeks following BM-DC treatment, Inguinal (IngLN) and Pancreatic-draining Lymph Node (PLN) CD4+ T cells were examined for Foxp3 expression (n=3). D. Representative dot plots of showing Foxp3 production in CD4+T cells.