Reactions Primary Normal Cells Manifesting Reaction Major Diagnostic Utility
Myeloperoxidase
(MPO)
Neutrophilseries+, eosinophils+, monocytes+/- L1, L2, L3 -
Sudan Black B
(SBB)
Neutrophilseries+, monocytes+/- L1, L2, L3 -
Non Specific Esterase (α-naphthyl acetate or α-naphthyl butyrate) Monocytes + (inhibited by sodium fluoride) L1, L2 (focal)+/-
L3-
 Periodic Acid Schiff (PAS) Neutrophil series, monocytes and lymphocytes + L1, L2 (70-75%) + (coarse granules or clumps in cytoplasm)
L3-
Acid Phosphatase (AP) Neutrophil series, monocytes, lymphocytes, megakaryocytes and platelets + L1, L2 (T cell)+
(strong focal paranuclear)
L3-
Table 3: Cytochemistry for ALL [28].