Figure 2: Causal relation between storage temperature, metabolic condition of platelets concentrates versus increasing in phagocytosis. Correlations between A) P-selectin expression vs binding percentage of PCs B) Annexin V binding to Phosphatidyl serine, and C) GPIb expression vs an increase in phagocytosis percentage in platelets from the same donor stored at room temperature (C22), ice 0°C(C0) and in refrigerator 4°C after metabolic suppression (MSP4) after 0, 40 min, 48h and 72 h storage. These figures indicates that the fresh PCs do not bind and phagocyted by macrophages but after prolonged storage step by step, time dependent, and receptors-condition dependently phagocyted by THP-1 macrophages in vitro. Hence, changes in Pselectin expression, PS exposure, and GPIb could be used as a qualification threshold to (dis-) qualify any random PCs prepared by any kind of method.