BM PB CB Mode of collection Usually in operation theatre, under general anesthesia, puncture of spinailiaca posterior, aspiration of up to 20 ml/kg body weight BM containing blood (corresponding to maximal 1500 ml or 25% of blood volume in children) anticoagulated with Citrate and / or heparin Mobilization of progenitor cells from bone marrow with eg; G-CSF pretreatment, apheresis of mononuclear cells into citrate anticoagulant After completed birth collection of blood from umbilical cord into citrate anticoagulant Typical processing steps Removal of larger particulate matter by filtration, depending on ABO compatibility plasma or red cell reduction; Selection and/or depletion of cells according to CD markers If needed volume reduction; selection and/or depletion of cells according to CD markers Depletion of red cells, volume reduction Storage Liquid storage after collection up to 72h at 2 to 6°C, or up to 48h at room temperature, or freezing within 72h after collection in a suitable cryoprotectant (e.g. 10% DMSO):≤‑140°C (above or in liquid nitrogen) several years as validated Liquid storage after collection up to 72h at 2 to 6°C, or up to 48h at room temperature, or freezing within 72h after collection in a suitable cryoprotectant (e.g. 10%DMSO): ≤‑140°C (above or in liquid nitrogen) several years as validated Freezing within 48h in a suitable cryopreservative solution (e.g. 10% DMSO): ≤‑140°C (above or in liquid nitrogen) several years as validated Dose rate autologous ≥2 x 108 nucleated cells/kg body weight ≥2 x 106 CD34+CD45+ cells/kg body weight ≥1.5 x 107 nucleated or CD45+cells/kg body weight Dose rate allogeneic ≥2 x 108 nucleated cells/kg body weight ≥4 x 106 CD34+CD45+cells/kg body weight ≥3 x 107 nucleated or CD45+cells/kg body weight

Table 1: Characteristics of HPC preparations for allogeneic transplantation in accordance with guidelines of the German Medical Association after consultation with the PEI.