Parameters Part of Plant used Method of Analysis Reference
Chlorophyll Fresh leaves Spectrophotometry Yasidia et al.,1971
Carotenoid and Lycopene Fresh leaves Spectrophotometry Zarkaria et al.,1979,Adsule &Ambadan,1979
Protein Fresh leaves and Vegetables Spectrophotometry Lowry et al.,1951
Starch Fresh leaves and Vegetables Colorimetry McCready et al .,1950
Ascorbic acid Vegetables Spectrophotometry Roe & Keuther,1953
Reducing sugars Vegetables Colorimetry Somogyi,1952
Total carbohydrates Vegetables Colorimetry Hedge & Hefreiter,1962
Total soluble sugars Vegetables Colorimetry Dubois,1956
Total phenol Vegetables Colorimetry Bray&Thorpe,1954
Calcium Vegetables Titrimetry Raghuramulu et al.,2003
Magnesium Vegetables Colorimetry Raghuramalu et al.,2003
Table 1: Methodology for analysis of Biochemical Parameters in Leaves and Fruits.