Developmental stage

Control (%)

 Seed extracts

Annona squamosa

 5% 10%

Calotropis procera

5% 10%

Larva

46.47 ±0.05

30.12**±0.16 (-35.17)

24.15*±0.65 (-48.01)

37.69*±0.47 (-18.89)

29.23* ±0.32 (-37.08)

Pupa

67.56 ±0.29

58.87*±3.46 (-12.85)

41.79*±4.21 (-38.14)

61.23ns±1.47 (-9.36)

49.34 ns±5.05 (-26.97)

Adult

195.98 ±0.93

157.65*±0.84 (-19.56)

85.57**±1.26 (-56.34)

162.56*±0.63 (-17.05)

112.76**±0.88 (-42.47)


The AChE activity in three developmental stages of the insect was assayed as indicated in Materials and Methods. Data were analyzed by Graphpad software, values given as mean ± SEM Values in parentheses are percent change over control. Significance (*) of data is shown in superscripts, nsP>0.05(non significant), *P<0.05(significant), **P<0.01(very significant), C-Control (0%), 5% & 10% are 1/20 and 1/10 of LC50.
Table 6: Effect of Annona squamosa and Calotropis procera ethanol extracts on AChE activity (nanomoles of ATI hydrolyzed/min/mg protein) of Musca domestica.